ELISA/assay

Dog Endothelin 1, ET-1 ELISA Kit

MBS704122

48-Strip-Wells

510 USD

ELISA Kit

Dog Endothelin 1, ET-1 ELISA Kit

endothelin 1

Dog Endothelin 1; ET-1 ELISA Kit; ET1; HDLCQ7; preproendothelin 1; endothelin 1

endothelin-1; Endothelin-1; endothelin-1; ET-1; preproendothelin-1; gene for endothelin; Preproendothelin-1; PPET1

EDN1

EDN1; EDN1; PPET1; ET-1; PPET1

EDN1_CANFA

Dog

202

This assay has high sensitivity and excellent specificity for detection of Dog EDN1. No significant cross-reactivity or interference between Dog EDN1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Endothelin-1(ET-1), a peptide of 21 amino acid residues, is the most potent vasoconstrictive substance known. Originally isolated from aortic endothelial cells, ET-1 is now known to be one of a family of three mammalian vasoactive peptides that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3). These related peptides differ from ET-1 at two and six amino acid residue positions, respectively. A fourth peptide, vasoactive intestinal contractor (VIC), is sometimes classified as rat ET-2. All members of the endothelin family contain two essential disulfide bridges and six conserved amino acid residues at the C-terminus. Additionally, all of the endothelin family members are synthesized initially as prepropolypeptides of approximately 200 amino acid residues encoded by separate genes. The biochemistry and biology of the endothelins have been the subject of several reviews. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ET-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

50950175

NP_001002956.1

NM_001002956.1

P13206

22,977 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1017189); G Alpha (q) Signalling Events Pathway (1017222); GPCR Downstream Signaling Pathway (1017218); GPCR Ligand Binding Pathway (1017188); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1017237); HIF-1 Signaling Pathway (695209); Melanogenesis Pathway (84142); Melanogenesis Pathway (504); Peptide Ligand-binding Receptors Pathway (1017190); Signal Transduction Pathway (1017095)

Function: Endothelins are endothelium-derived vasoconstrictor peptides.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800