Human Dentin Matrix Protein 1, DMP1 ELISA Kit | MyBioSource MBS704117 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Dentin Matrix Protein 1, DMP1 ELISA Kit

catalog :

MBS704117

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704117

products type :

ELISA Kit

products full name :

Human Dentin Matrix Protein 1, DMP1 ELISA Kit

products short name :

dentin matrix acidic phosphoprotein 1

products name syn :

Human Dentin Matrix Protein 1 (DMP1) ELISA kit; ARHP; ARHR; DMP-1; dentin matrix protein 1; dentin matrix acidic phosphoprotein 1

other names :

dentin matrix acidic phosphoprotein 1 isoform 2; Dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein 1; dentin matrix protein 1; dentin matrix acidic phosphoprotein 1

products gene name :

DMP1

other gene names :

DMP1; DMP1; ARHP; ARHR; DMP-1; DMP-1; Dentin matrix protein 1

uniprot entry name :

DMP1_HUMAN

reactivity :

Human

sequence length :

497

specificity :

This assay has high sensitivity and excellent specificity for detection of Human DMP1. No significant cross-reactivity or interference between Human DMP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Biomineralization

products description :

For samples: For the quantitative determination of human dentin matrix protein 1 (DMP1) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

121256621

ncbi acc num :

NP_001073380.1

ncbi gb acc num :

NM_001079911.2

uniprot acc num :

Q13316

ncbi mol weight :

55,782 Da

ncbi pathways :

ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.1 PublicationManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage