ELISA/assay

Rabbit High mobility group protein B1, HMGB-1 ELISA Kit

MBS704090

96 Strip Wells

915 USD

ELISA Kit

Rabbit High mobility group protein B1, HMGB-1 ELISA Kit

High mobility group protein B1, HMGB-1

Rabbit High mobility group protein B1; HMGB-1 ELISA Kit; High mobility group protein B1; HMGB-1

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; high mobility group box 1; High mobility group protein 1; HMG-1

HMGB-1

HMGB1; HMGB1; HMG1; HMG3; SBP-1; HMG1; HMG-1

HMGB1_HUMAN

Rabbit

215

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma. Detection Range: 5.7 ng/ml-100 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 3.57 ng/ml

Sample Volume: 50-100ul

Introduction: High Mobility Group Box 1 protein (HMGB1) is a non-histone chomosomal protein that functions in endotoxin lethality. It is released from activated macrophages and is present at elevated levels in the serum of sepsis patients. HMGB1 was also reported to interact with RAGE (receptor for advanced glycation end products) and to influence neuron development. High mobility group box-1 protein (HMGB1) has been known for many years as a nuclear chromosomal protein. Its role as a pro-inflammatory cytokine in sepsis and rheumatoid arthritis has been described recently. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMGB-1. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for HMGB-1 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain HMGB-1, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HMGB-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4504425

NP_002119.1

NM_002128.4

P09429

24,894 Da

Activated TLR4 Signalling Pathway 106400!!Activation Of DNA Fragmentation Factor Pathway 105683!!Advanced Glycosylation Endproduct Receptor Signaling Pathway 187092!!Apoptosis Pathway 105648!!Apoptosis Induced DNA Fragmentation Pathway 105682!!Apoptotic Execution Phase Pathway 105677!!Base Excision Repair Pathway 83043!!Base Excision Repair Pathway 451!!Cytosolic Sensors Of Pathogen-associated DNA Pathway 576255!!DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway 833822

Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells

96 Strip Wells

915 USD