ELISA/assay

Rat Aspartate Aminotransferase, AST ELISA Kit

MBS704087

48-Strip-Wells

565 USD

ELISA Kit

Rat Aspartate Aminotransferase, AST ELISA Kit

glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)

Rat Aspartate Aminotransferase (AST) ELISA kit; GIG18; aspartate aminotransferase 1; growth-inhibiting protein 18; glutamic-oxaloacetic transaminase 1; soluble (aspartate aminotransferase 1)

Aspartate aminotransferase, cytoplasmic; Aspartate aminotransferase, cytoplasmic; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A

GOT1

GOT1; cAspAT; cCAT

AATC_RABIT

Rat

31

This assay has high sensitivity and excellent specificity for detection of Rat GOT1. No significant cross-reactivity or interference between Rat GOT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 3.12 mIU/ml-200 mIU/ml. Sensitivity: 0.78 mIU/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AST is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AST bound in the initial step. The color development is stopped and the intensity of the color is measured.

126302509

P12343.2

P12343

3,427 Da

Function: Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain . Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate. Cofactor: Pyridoxal phosphate.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150