ELISA/assay

Human Thyroxine-Binding Globulin, TBG ELISA Kit

MBS704076

48-Strip-Wells

565 USD

ELISA Kit

Human Thyroxine-Binding Globulin, TBG ELISA Kit

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) , member 7

Human Thyroxine-Binding Globulin; TBG ELISA Kit; RP1-82J11.2; TBG; OTTHUMP00000023779; alpha-1 antiproteinase; antitrypsin; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 7; serine (or cysteine) prote; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 7

thyroxine-binding globulin; Thyroxine-binding globulin; thyroxine-binding globulin; serpin A7; T4-binding globulin; thyroxin-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; Serpin A7; T4-binding globulin

SERPINA7

SERPINA7; SERPINA7; TBG; TBG

THBG_HUMAN

Human

415

This assay has high sensitivity and excellent specificity for detection of Human SERPINA7. No significant cross-reactivity or interference between Human SERPINA7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 12.5ng/ml-200ng/ml. Sensitivity: 6.25 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to TBG has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TBG and incubated. A competitive inhibition reaction is launched between TBG (Standards or samples) and Biotin-conjugated TBG with the pre-coated antibody specific for TBG. The more amount of TBG in samples, the less antibody bound by Biotin-conjugated TBG. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TBG in the sample. The color development is stopped and the intensity of the color is measured.

205277441

NP_000345.2

NM_000354.5

P05543

46,325 Da

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

Function: Major thyroid hormone transport protein in serum.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150