Human visfatin ELISA Kit | MyBioSource MBS704072 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human visfatin ELISA Kit

catalog :

MBS704072

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704072

products type :

ELISA Kit

products full name :

Human visfatin ELISA Kit

products short name :

nicotinamide phosphoribosyltransferase

products name syn :

Human visfatin ELISA Kit; 1110035O14Rik; DKFZp666B131; MGC117256; PBEF; PBEF1; VF; VISFATIN; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; nicotinamide phosphoribosyltransferase

other names :

nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; nicotinamide phosphoribosyltransferase; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

products gene name :

NAMPT

other gene names :

NAMPT; NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik; PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor

uniprot entry name :

NAMPT_HUMAN

reactivity :

Human

sequence length :

491

specificity :

This assay has high sensitivity and excellent specificity for detection of Human NAMPT. No significant cross-reactivity or interference between Human NAMPT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Alkaloid biosynthesis and metabolism. Protein Biological Process 3: Pyridine nucleotide biosynthesis

products description :

For Samples For the quantitative determination of human visfatin concentrations in serum, plasma, urine, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for visfatin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any visfatin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for visfatin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of visfatin bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5031977

ncbi acc num :

NP_005737.1

ncbi gb acc num :

NM_005746.2

uniprot acc num :

P43490

ncbi mol weight :

55,521 Da

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003)

ncbi summary :

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

uniprot summary :

Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function.1 PublicationManual assertion based on experiment in:Ref.11. Catalytic activity: Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate. Enzyme regulation: Inhibited by FK866. FK866 competes for the same binding site as nicotinamide, but due to its very low dissociation rate, it is essentially an irreversible inhibitor.1 PublicationManual assertion based on experiment in:Ref.12

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage