Rat Complement fragment 4a, C4a ELISA Kit | MyBioSource MBS704067 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Complement fragment 4a, C4a ELISA Kit

catalog :

MBS704067

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS704067

products type :

ELISA Kit

products full name :

Rat Complement fragment 4a, C4a ELISA Kit

products short name :

Complement fragment 4a, C4a

products name syn :

Rat Complement fragment 4a; C4a ELISA Kit; Complement fragment 4a; C4a

other names :

complement C4-A isoform 1 preproprotein; Complement C4-A; complement C4-A; acidic C4; C4A anaphylatoxin; Rodgers form of C4; acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2; complement component 4A (Rodgers blood group); Acidic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2Cleaved into the following 6 chains:Complement C4 beta chain; Complement C4-A alpha chain; C4a anaphylatoxin; C4b-A; C4d-A; Complement C4 gamma chain

products gene name :

C4a

other gene names :

C4A; C4A; C4; RG; C4S; CO4; C4A2; C4A3; C4A4; C4A6; C4AD; CPAMD2; CO4; CPAMD2

uniprot entry name :

CO4A_HUMAN

reactivity :

Rat

sequence length :

1744

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 ng/ml-4000 ng/ml. Sensitivity: 15.6 ng/ml

other info2 :

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4a bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

67190748

ncbi acc num :

NP_009224.2

ncbi gb acc num :

NM_007293.2

uniprot acc num :

P0C0L4

ncbi mol weight :

187,704 Da

ncbi pathways :

Activation Of C3 And C5 Pathway (106412); Allograft Rejection Pathway (920963); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); Immune System Pathway (106386); Initial Triggering Of Complement Pathway (106406); Innate Immune System Pathway (106387); Pertussis Pathway (218111)

ncbi summary :

This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

uniprot summary :

Function: Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.2 PublicationsManual assertion based on experiment in:Ref.19

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage