ELISA/assay

Mouse thrombospondin 1, TSP-1 ELISA Kit

MBS704061

48-Strip-Wells

590 USD

ELISA Kit

Mouse thrombospondin 1, TSP-1 ELISA Kit

thrombospondin 1

Mouse thrombospondin 1; TSP-1 ELISA Kit; THBS; THBS-1; TSP; TSP-1; TSP1; thrombospondin-1p180; thrombospondin 1

Thrombospondin-1; Thrombospondin-1; thrombospondin-1; thrombospondin 1

THBS1

Thbs1; Thbs1; TSP1; TSP-1; tbsp1; Thbs-1; Tsp1

TSP1_MOUSE

Mouse

1170

This assay has high sensitivity and excellent specificity for detection of mouse TSP-1. No significant cross-reactivity or interference between mouse TSP-1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: 1.95 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TSP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

549134

P35441.1

P35441

129,647 Da

Bladder Cancer Pathway (83308); Bladder Cancer Pathway (527); ECM-receptor Interaction Pathway (83265); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (1001015); Focal Adhesion Pathway (198353); Focal Adhesion Pathway (83264); Focal Adhesion Pathway (478); Hemostasis Pathway (1001295); Inflammatory Response Pathway (198280)

The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Function: Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp. Ligand for CD36 mediating antiangiogenic properties Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors.1 PublicationManual assertion based on experiment in:Ref.6

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440