ELISA/assay

Human glial fibrillary acidic protein, GFAP ELISA Kit

MBS704044

48-Strip-Wells

510 USD

ELISA Kit

Human glial fibrillary acidic protein, GFAP ELISA Kit

glial fibrillary acidic protein

Human glial fibrillary acidic protein; GFAP ELISA Kit; FLJ45472; ; glial fibrillary acidic protein

glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein

GFAP

GFAP; GFAP; GFAP

GFAP_HUMAN

Human

431

This assay has high sensitivity and excellent specificity for detection of human GFAP. No significant cross-reactivity or interference between human GFAP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human GFAP is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GFAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GFAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GFAP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GFAP bound in the initial step. The color development is stopped and the intensity of the color is measured.

196115290

NP_001124491.1

NM_001131019.2

P14136

49,880 Da

Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (530744); Signal Transduction Pathway (477114); Signaling By ERBB4 Pathway (530741); Spinal Cord Injury Pathway (739007)

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Function: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800