ELISA/assay

Human Annexin I , ANX-I  ELISA Kit

MBS704042

48-Strip-Wells

565 USD

ELISA Kit

Human Annexin I , ANX-I  ELISA Kit

annexin A1

Human Annexin I (ANX-I) ELISA Kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; annexin A1

annexin A1; Annexin A1; annexin A1; p35; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; annexin I (lipocortin I); phospholipase A2 inhibitory protein; annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35

ANXA1

ANXA1; ANXA1; ANX1; LPC1; ANX1; LPC1

ANXA1_HUMAN

Human

346

This assay has high sensitivity and excellent specificity for detection of human ANX-I. No significant cross-reactivity or interference between human ANX-I and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANX-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANX-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANX-I is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANX-I bound in the initial step. The color development is stopped and the intensity of the color is measured.

4502101

NP_000691.1

NM_000700.1

P04083

38,714 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Integrated Breast Cancer Pathway (219801); Integrated Pancreatic Cancer Pathway (711360); Peptide Ligand-binding Receptors Pathway (106358)

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]

Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150