ELISA/assay

Bovine milk fat globule-EGF factor 8 protein, MFGE8 ELISA Kit

MBS704034

96 Strip Wells

895 USD

ELISA Kit

Bovine milk fat globule-EGF factor 8 protein, MFGE8 ELISA Kit

milk fat globule-EGF factor 8 protein

Bovine milk fat globule-EGF factor 8 protein (MFGE8) ELISA Kit; BA46; EDIL1; HsT19888; MFG-E8; OAcGD3S; SED1; SPAG10; hP47; O-acetyl disialoganglioside synthase; lactadherin; medin; sperm associated antigen 10; sperm surface protein hP47; milk fat globule-EGF factor 8 protein

lactadherin; Lactadherin; lactadherin; BP47; MFGM; SED1; MFG-E8; MGP57/53; components 15/16; PAS-6/PAS-7 glycoprotein; sperm surface protein SP47; milk fat globule-EGF factor 8 protein<; BP47; Components 15/16; MFGM; MGP57/53; Milk fat globule-EGF factor 8; MFG-E8; PAS-6/PAS-7 glycoprotein; SED1; Sperm surface protein SP47

MFGE8

MFGE8; MFGE8; MFG-E8

MFGM_BOVIN

Bovine

427

This assay has high sensitivity and excellent specificity for detection of bovine MFGE8. No significant cross-reactivity or interference between bovine MFGE8 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of bovine milk fat globule-EGF factor 8 protein (MFGE8) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.78 ng/ml-50 ng/ml.

Sensitivity: The minimum detectable dose of bovine MFGE8 is typically less than 0.2 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFGE8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFGE8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFGE8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFGE8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

41386719

NP_788783.1

NM_176610.1

Q95114

47,411 Da

Amyloids Pathway 1008671!!Disease Pathway 942846

Function: Contributes to phagocytic removal of apoptotic cells in many tissues. Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.1 PublicationManual assertion based on experiment in:Ref.6

96 Strip Wells

895 USD