catalog number :
MBS704029
products type :
ELISA Kit
products full name :
Guinea Pig interleukin 10, IL-10 ELISA Kit
products short name :
interleukin 10
products name syn :
Guinea pig interleukin 10; IL-10 ELISA Kit; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; interleukin 10
other names :
Interleukin-10; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF
products gene name :
IL10
other gene names :
IL10; IL-10; CSIF
uniprot entry name :
IL10_CAVPO
specificity :
This assay has high sensitivity and excellent specificity for detection of guinea pig IL-10. No significant cross-reactivity or interference between guinea pig IL-10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of guinea pig IL-10 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
20,238 Da
uniprot summary :
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells
size4 :
10x96-Strip-Wells