ELISA/assay

Rat Bcl-2-like protein 1, Bcl2-L-1/Bcl-X ELISA Kit

MBS704027

96 Strip Wells

850 USD

ELISA Kit

Rat Bcl-2-like protein 1, Bcl2-L-1/Bcl-X ELISA Kit

BCL2-like 1

Rat Bcl-2-like protein 1 (Bcl2-L-1/Bcl-X) ELISA Kit; BCL-XL/S; BCL2L; BCLX; Bcl-X; DKFZp781P2092; bcl-xL; bcl-xS; OTTHUMP00000030550; BCL2-like 1

bcl-2-like protein 1 isoform 2; Bcl-2-like protein 1; bcl-2-like protein 1; bcl2-L-1; B cell lymphoma 2 like; B cell lymphoma like X; apoptosis regulator Bcl-X; Bcl2-like 1; Apoptosis regulator Bcl-X

BCL2L1

Bcl2l1; Bcl2l1; Bclx; Bcl2l; bcl-X; Bcl-xl; Bclx; Blc2l; Bcl2-L-1

B2CL1_RAT

Rat

233

This assay has high sensitivity and excellent specificity for detection of rat Bcl2-L1/Bcl-X. No significant cross-reactivity or interference between rat Bcl2-L1/Bcl-X and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat Bcl-2-like protein 1 (Bcl2-L-1/Bcl-X) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 46.88 pg/ml -3000 pg/ml.

Sensitivity: The minimum detectable dose of rat Bcl2-L1/Bcl-X is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Bcl2-L-1/Bcl-X has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Bcl2-L-1/Bcl-X present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Bcl2-L-1/Bcl-X is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Bcl2-L-1/Bcl-X bound in the initial step. The color development is stopped and the intensity of the color is measured.

75992930

NP_001028842.1

NM_001033670.1

P53563

26,158 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway 83491!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!Apoptosis Pathway 219785!!Apoptosis Pathway 83452!!Apoptosis Pathway 470!!Chronic Myeloid Leukemia Pathway 83504!!Chronic Myeloid Leukemia Pathway 528!!HTLV-I Infection Pathway 373894!!HTLV-I Infection Pathway 373889!!IL-3 Signaling Pathway 198504

inhibits neuronal apoptosis; may provide neuroprotection against ischemic brian injury [RGD, Feb 2006]

Function: Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.5 PublicationsManual assertion based on experiment in:Ref.3

96 Strip Wells

850 USD