ELISA/assay

Pig monocyte chemotactic protein 1, MCP1 ELISA Kit

MBS704018

48-Strip-Wells

565 USD

ELISA Kit

Pig monocyte chemotactic protein 1, MCP1 ELISA Kit

chemokine (C-C motif) ligand 2

Pig monocyte chemotactic protein 1; MCP1 ELISA Kit; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; MGC9434; SCYA2; SMC-CF; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine; chemokine (C-C motif) ligand 2

C-C motif chemokine 2; C-C motif chemokine 2; C-C motif chemokine 2; small-inducible cytokine A2; monocyte chemotactic protein 1; monocyte chemoattractant protein 1; Monocyte chemoattractant protein 1; Monocyte chemotactic protein 1; MCP-1; Small-inducible cytokine A2

CCL2

CCL2; CCL2; MCP-1; SCYA2; MCP-1

CCL2_PIG

Pig

99

This assay has high sensitivity and excellent specificity for detection of pig MCP1. No significant cross-reactivity or interference between pig MCP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of pig MCP1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

47523512

NP_999379.1

NM_214214.1

P42831

10,976 Da

ATF4 Activates Genes Pathway (1014428); Chagas Disease (American Trypanosomiasis) Pathway (147807); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chemokine Receptors Bind Chemokines Pathway (1013338); Chemokine Signaling Pathway (98758); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1013336); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); GPCR Ligand Binding Pathway (1013335)

Function: Chemotactic factor that attracts monocytes, but not neutrophils.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150