ELISA/assay

Mouse Platelet-Derived Growth Factor Soluble Receptor alpha, PDGFsR-alpha ELISA Kit

MBS704014

48-Strip-Wells

590 USD

ELISA Kit

Mouse Platelet-Derived Growth Factor Soluble Receptor alpha, PDGFsR-alpha ELISA Kit

platelet-derived growth factor receptor, alpha polypeptide

Mouse Platelet-Derived Growth Factor Soluble Receptor alpha; PDGFsR-alpha ELISA kit; CD140A; MGC74795; PDGFR2; RHEPDGFRA; CD140 antigen-like family member A; PDGF-R-alpha; PDGFRA/BCR fusion; platelet-derived growth factor receptor alpha; rearranged-in-hypereosinophilia-platelet derived; platelet-derived growth factor receptor; alpha polypeptide

platelet-derived growth factor receptor alpha; Platelet-derived growth factor receptor alpha; platelet-derived growth factor receptor alpha; PDGFR-alpha; PDGF-R-alpha; PDGF alpha chain; CD140 antigen-like family member A; platelet-derived growth factor alpha receptor; alpha-type platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); platelet derived growth factor receptor, alpha polypeptide; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; Platelet-derived growth factor alpha receptor; CD_antigen: CD140a

PDGFRA

Pdgfra; Pdgfra; CD140a; Pdgfr-2; AI115593; PDGF-R-alpha; PDGFR-alpha

PGFRA_MOUSE

Mouse

1089

This assay has high sensitivity and excellent specificity for detection of mouse PDGFsR-alpha. No significant cross-reactivity or interference between mouse PDGFsR-alpha and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of mouse PDGFsR-alpha is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGFsR-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGFsR-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGFsR-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGFsR-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

134032016

NP_001076785.1

NM_001083316.1

P26618

122,683 Da

Adaptive Immune System Pathway (971210); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Constitutive PI3K/AKT Signaling In Cancer Pathway (1000676); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214); Disease Pathway (970606); Downstream Signal Transduction Pathway (971469)

Function: Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca2+ and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.4 PublicationsManual assertion based on experiment in:Ref.5. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Present in an inactive conformation in the absence of bound ligand. Binding of PDGFA and/or PDGFB leads to dimerization and activation by autophosphorylation on tyrosine residues. Inhibited by imatinib, nilotinib and sorafenib

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440