catalog number :
MBS703996
products type :
ELISA Kit
products full name :
Bovine Interleukin 1beta, IL- 1beta ELISA Kit
products short name :
interleukin 1, beta
products name syn :
Bovine Interleukin 1beta (IL- 1beta) ELISA kit; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1; beta
other names :
interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1, beta
products gene name :
IL1B
other gene names :
IL1B; IL1B; IL-1 beta
uniprot entry name :
IL1B_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine IL-1beta. No significant cross-reactivity or interference between bovine IL-1beta and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 20 pg/ml-4000 pg/ml. Sensitivity: The minimum detectable dose of bovine IL-1beta is typically less than 10 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine IL-1beta concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for IL-1beta and Horseradish Peroxidase (HRP) conjugated IL-1beta. The competitive inhibition reaction is launched between with HRP labeled IL-1beta and unlabeled IL-1beta with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IL-1beta in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776518.1
ncbi gb acc num :
NM_174093.1
ncbi mol weight :
30,760 Da
ncbi pathways :
African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (84336); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Apoptosis Pathway (84299); Apoptosis Pathway (470); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795)
uniprot summary :
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
size4 :
10x96-Strip-Wells