ELISA/assay

Human Bone morphogenetic protein 7, BMP-7 ELISA Kit

MBS703989

96 Strip Wells

575 USD

ELISA Kit

Human Bone morphogenetic protein 7, BMP-7 ELISA Kit

bone morphogenetic protein 7

Human Bone morphogenetic protein 7; BMP-7 ELISA Kit; OP-1; osteogenic protein 1; bone morphogenetic protein 7

bone morphogenetic protein 7; Bone morphogenetic protein 7; bone morphogenetic protein 7; osteogenic protein 1; bone morphogenetic protein 7; Osteogenic protein 1; OP-1; INN: Eptotermin alfa

BMP7

BMP7; BMP7; OP-1; OP1; BMP-7; OP-1

BMP7_HUMAN

Human

431

This assay has high sensitivity and excellent specificity for detection of Human BMP7. No significant cross-reactivity or interference between Human BMP7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 15.6 pg/ml -1000 pg/ml. T. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Chondrogenesis

Introduction: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.[2] BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes.[3] It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.[2] BMP7 has been discovered to be crucial in the determination of ventral-dorsal organization in zebrafish. BMP7 causes the expression of ventral phenotypes while its complete inhibition creates a dorsal phenotype. Moreover, BMP7 is eventually partially "turned off" in embryonic development in order to create the dorsal parts of the organism.[4] In many early developmental experiments using zebrafish, scientists used caBMPR (constitutively active) and tBMP (truncated receptor) to determine the effect of BMP7 in embryogensis. They found that the constitutively active, which causes BMP to be expressed everywhere creates a ventralized phenotype, whereas truncated, dorsalized.FOR . Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP-7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BMP-7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BMP-7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BMP-7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4502427

NP_001710.1

NM_001719.2

P18075

49,313 Da

ALK2 Signaling Events Pathway 137983!!BMP Receptor Signaling Pathway 137948!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Elastic Fibre Formation Pathway 730310!!Endochondral Ossification Pathway 198812!!Extracellular Matrix Organization Pathway 576262!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388!!Molecules Associated With Elastic Fibres Pathway 730311

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development and possible bone inductive activity. [provided by RefSeq, Jul 2008]

Function: Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

96 Strip Wells

575 USD