ELISA/assay

Pig Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

MBS703976

48-Strip-Wells

465 USD

ELISA Kit

Pig Vascular Endothelial cell Growth Factor, VEGF ELISA Kit

vascular endothelial growth factor A

Pig Vascular Endothelial cell Growth Factor; VEGF ELISA KIT; RP1-261G23.1; MGC70609; MVCD1; VEGF; VEGF-A; VPF; vascular endothelial growth factor isoform VEGF165; vascular permeability factor; vascular endothelial growth factor A

vascular endothelial growth factor A; Vascular endothelial growth factor A; vascular endothelial growth factor A; VPF; VEGF-A; vascular permeability factor; Vascular permeability factor; VPF

VEGFA

VEGFA; VEGFA; VEGF; VEGF; VEGF-A; VPF

VEGFA_PIG

Pig

190

This assay has high sensitivity and excellent specificity for detection of pig VEGF. No significant cross-reactivity or interference between pig VEGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.69 pg/ml -300 pg/ml. Sensitivity: The minimum detectable dose of pig VEGF is typically less than 1.17 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VEGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

47522980

NP_999249.1

NM_214084.1

P49151

22,368 Da

Axon Guidance Pathway (1014044); Bladder Cancer Pathway (84522); Bladder Cancer Pathway (527); Cellular Response To Hypoxia Pathway (1013761); Cellular Responses To Stress Pathway (1013760); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Developmental Biology Pathway (1014043); EPH-Ephrin Signaling Pathway (1014071); EPHA-mediated Growth Cone Collapse Pathway (1014072)

Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin

48-Strip-Wells

465 USD

96-Strip-Wells

655

5x96-Strip-Wells

2455

10x96-Strip-Wells

4595