catalog number :
MBS703971
products type :
ELISA Kit
products full name :
Human beta-defensins 1 ELISA Kit
products short name :
defensin, beta 1
products name syn :
Human beta-defensins 1 ELISA Kit; BD1; DEFB-1; DEFB101; HBD1; MGC51822; beta-defensin-1; defensin; beta 1
other names :
beta-defensin 1 preproprotein; Beta-defensin 1; beta-defensin 1; BD-1; beta-defensin-1; defensin, beta 1; Defensin, beta 1
products gene name :
DEFB1
other gene names :
DEFB1; DEFB1; BD1; HBD1; DEFB-1; DEFB101; BD1; HBD1; BD-1; hBD-1
uniprot entry name :
DEFB1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human beta-defensins 1. No significant cross-reactivity or interference between human beta-defensins 1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of human beta-defensins is typically less than 0.78 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_005209.1
ncbi gb acc num :
NM_005218.3
ncbi mol weight :
7,420 Da
ncbi pathways :
Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)
ncbi summary :
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Has bactericidal activity
size4 :
10x96-Strip-Wells