catalog number :
MBS703960
products type :
ELISA Kit
products full name :
Canine Matrix metalloproteinase 13, MMP-13 ELISA Kit
products short name :
[Matrix metalloproteinase 13, MMP-13]
products name syn :
[Canine Matrix metalloproteinase 13; MMP-13 ELISA kit; Matrix metalloproteinase 13; MMP-13]
other names :
[MMP-13, partial; MMP-13; MMP-13]
products gene name :
[MMP-13]
uniprot entry name :
Q9BGL6_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of canine MMP-13. No significant cross-reactivity or interference between canine MMP-13 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: Typically less than 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAG59845.1
ncbi mol weight :
16,463 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells