ELISA/assay

Rat Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

MBS703940

96 Strip Wells

915 USD

ELISA Kit

Rat Peroxisome Proliferator-activated receptor gamma, PPAR-gamma ELISA Kit

peroxisome proliferator-activated receptor gamma

Rat Peroxisome Proliferator-activated receptor gamma; PPAR-gamma ELISA Kit; CIMT1; GLM1; NR1C3; PPARG1; PPARG2; PPARgamma; OTTHUMP00000185030; OTTHUMP00000185033; PPAR gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferative activated receptor gamma; peroxi; peroxisome proliferator-activated receptor gamma

Peroxisome proliferator-activated receptor gamma; Peroxisome proliferator-activated receptor gamma; Nuclear receptor subfamily 1 group C member 3

PPARG

PPARG; NR1C3; PPAR-gamma

PPARG_RABIT

Rat

475

This assay has high sensitivity and excellent specificity for detection of Rat PPARG. No significant cross-reactivity or interference between Rat PPARG and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Introduction: Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase & cytochrome P450 (CYP450) A6 through interaction with specific response elements. The PPAR gamma 2 isoform appears to be induced very early in the differentiation of several cultured adipocyte cell lines, and has been suggested to be a dominant regulator of the murine P2 (aP2) gene which encodes an intracellular lipid binding protein which is expressed only in adipose cells. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with RXR alpha. Defects in PPAR gamma can lead to type 2 insulin-resistant diabetes and hyptertension and could play a role in the genetic predisposition to obesity. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPAR-gamma. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PPAR-gamma and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PPAR-gamma, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PPAR-gamma in the samples is then determined by comparing the O.D. of the samples to the standard curve.

3024414

O19052.1

O19052

54,365 Da

Developmental Biology Pathway 581958!!Gene Expression Pathway 581891!!Generic Transcription Pathway 581892!!Huntington's Disease Pathway 84339!!Huntington's Disease Pathway 512!!Nuclear Receptor Transcription Pathway 581893!!Nuclear Receptors Pathway 198578!!Nuclear Receptors In Lipid Metabolism And Toxicity Pathway 198560!!Osteoclast Differentiation Pathway 193144!!Osteoclast Differentiation Pathway 193096

Function: Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses . Enzyme regulation: PDPK1 activates its transcriptional activity independently of its kinase activity

96 Strip Wells

915 USD