ELISA/assay

Mouse Bone morphogenetic protein 4, BMP-4 ELISA Kit

MBS703931

48-Strip-Wells

405 USD

ELISA Kit

Mouse Bone morphogenetic protein 4, BMP-4 ELISA Kit

bone morphogenetic protein 4

Mouse Bone morphogenetic protein 4; BMP-4 ELISA Kit; BMP2B; BMP2B1; MCOPS6; OFC11; ZYME; bone morphogenetic protein 2B; bone morphogenetic protein 4

bone morphogenetic protein 4; Bone morphogenetic protein 4; bone morphogenetic protein 4; bone morphogenetic protein 2B; bone morphogenetic protein 4; Bone morphogenetic protein 2B; BMP-2B

BMP4

Bmp4; Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1; Bmp-4; Dvr-4; BMP-4; BMP-2B

BMP4_MOUSE

Mouse

408

This assay has high sensitivity and excellent specificity for detection of mouse BMP-4. No significant cross-reactivity or interference between mouse BMP-4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 pg/ml -40 pg/ml. Sensitivity: The minimum detectable dose of mouse BMP-4 is typically less than 0.156 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

121949823

NP_031580.2

NM_007554.2

P21275

46,497 Da

Adipogenesis Pathway (198299); Basal Cell Carcinoma Pathway (83306); Basal Cell Carcinoma Pathway (525); ESC Pluripotency Pathways (198374); Elastic Fibre Formation Pathway (1001022); Extracellular Matrix Organization Pathway (1001015); Heart Development Pathway (672437); Hedgehog Signaling Pathway (83260); Hedgehog Signaling Pathway (474); Hippo Signaling Pathway (749786)

Function: Induces cartilage and bone formation. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction.3 PublicationsManual assertion based on experiment in:Ref.9

48-Strip-Wells

405 USD

96-Strip-Wells

570

5x96-Strip-Wells

2240

10x96-Strip-Wells

4185