catalog number :
MBS703925
products type :
ELISA Kit
products full name :
Canine Matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit
products short name :
Matrix metalloproteinase 2/Gelatinase A, MMP-2
products name syn :
Canine Matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA kit; Matrix metalloproteinase 2/Gelatinase A; MMP-2
other names :
MMP-2, partial; MMP-2; 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase 2; matrix metalloproteinase-2; matrix metallopeptidase 2; MMP-2; Matrix metalloproteinase 2
products gene name :
MMP2/Gelatinase A
other gene names :
Mmp2; Mmp2; MMP-2
uniprot entry name :
Q9JHC2_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of canine MMP-2. No significant cross-reactivity or interference between canine MMP-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.69 pg/ml-300 pg/ml. Sensitivity: The minimum detectable dose of canine MMP-2 is typically less than 1.17 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAF71618.1
ncbi mol weight :
3,196 Da
ncbi pathways :
Bladder Cancer Pathway (83503); Bladder Cancer Pathway (527); Estrogen Signaling Pathway (799190); Estrogen Signaling Pathway (799197); GnRH Signaling Pathway (83483); GnRH Signaling Pathway (502); IL-3 Signaling Pathway (198504); Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Matrix Metalloproteinases Pathway (198492)
ncbi summary :
plays important roles in wound healing, angiogenesis, platelet aggregation, and tumor metastasis; mediates the response of the intrinsic glomerular mesangial cell to inflammatory stimuli [RGD, Feb 2006]
size4 :
10x96-Strip-Wells