catalog number :
MBS703924
products type :
ELISA Kit
products full name :
Chicken Heat Shock Protein 70, Hsp-70 ELISA Kit
products short name :
Heat Shock Protein 70, Hsp-70
products name syn :
Chicken Heat Shock Protein 70; Hsp-70 ELISA Kit; Heat Shock Protein 70; Hsp-70
other names :
HSP70; HSP70; HSP70
products gene name :
HSP70
uniprot entry name :
Q40058_HORVU
specificity :
This assay has high sensitivity and excellent specificity for detection of chicken Hsp-70. No significant cross-reactivity or interference between chicken Hsp-70 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 0.25 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of chicken Hsp-70 is typically less than 0.125 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest chicken Hsp-70 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for Hsp-70 and Horseradish Peroxidase (HRP) conjugated Hsp-70. The competitive inhibition reaction is launched between with HRP labeled Hsp-70 and unlabeled Hsp-70 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Hsp-70 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAA62325.1
ncbi mol weight :
67,016 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells