catalog number :
MBS703912
products type :
ELISA Kit
products full name :
Dog beta2-microglobulin, BMG/beta2-MG ELISA Kit
products short name :
beta-2-microglobulin
products name syn :
Dog beta2-microglobulin; BMG/beta2-MG ELISA Kit; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
other names :
Beta-2-microglobulin, partial; Beta-2-microglobulin
uniprot entry name :
B2MG_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog B2M. No significant cross-reactivity or interference between Dog B2M and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.78 ug/ml-50 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 0.2 ug/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta2-MG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta2-MG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta2-MG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta2-MG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
4,842 Da
ncbi pathways :
Adaptive Immune System Pathway 366160!!Amyloids Pathway 366238!!Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway 366163!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Antigen Processing-Cross Presentation Pathway 477122!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Cytokine Signaling In Immune System Pathway 366171!!Disease Pathway 530764!!Downstream Signaling In Naive CD8+ T Cells Pathway 138018
uniprot summary :
Function: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system