catalog number :
MBS703902
products type :
ELISA Kit
products full name :
Human A Disintegrin And Metalloprotease 9, ADAM9 ELISA Kit
products short name :
ADAM metallopeptidase domain 9 (meltrin gamma)
products name syn :
Human A Disintegrin And Metalloprotease 9; ADAM9 ELISA Kit; CORD9; KIAA0021; MCMP; MDC9; Mltng; ADAM metallopeptidase domain 9; a disintegrin and metalloproteinase domain 9 (meltrin gamma) ; cellular disintegrin-related protein; meltrin gamma; myeloma cell metall; ADAM metallopeptidase domain 9 (meltrin gamma)
other names :
disintegrin and metalloproteinase domain-containing protein 9; Disintegrin and metalloproteinase domain-containing protein 9; disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9; ADAM metallopeptidase domain 9; Cellular disintegrin-related protein; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; Myeloma cell metalloproteinase
products gene name :
ADAM9
other gene names :
ADAM9; ADAM9; MCMP; MDC9; CORD9; Mltng; KIAA0021; MCMP; MDC9; MLTNG; ADAM 9
uniprot entry name :
ADAM9_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human ADAM9. No significant cross-reactivity or interference between Human ADAM9 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates and ascitic fluid. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM9. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for ADAM9 to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM9, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_003807.1
ncbi gb acc num :
NM_003816.2
ncbi mol weight :
90,556 Da
ncbi pathways :
Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)
ncbi summary :
This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]
uniprot summary :
Function: Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.1 PublicationManual assertion based on experiment in:Ref.3. Cofactor: Binds 1 zinc ion per subunit Inferred.
size4 :
10x96-Strip-Wells