catalog number :
MBS703899
products type :
ELISA Kit
products full name :
Bovine Interleukin 12, IL-12 ELISA Kit
products short name :
interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
products name syn :
Bovine Interleukin 12; IL-12 ELISA Kit; CLMF; IL-12A; NFSK; NKSF1; P35; IL-12; subunit p35; NF cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1; p35; interleukin 12; p35; interleukin 12A; interleukin-12 alpha chain; nat; interleukin 12A (natural killer cell stimulatory factor 1; cytotoxic lymphocyte maturation factor 1; p35)
other names :
interleukin-12 subunit alpha; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; interleukin 12 35kDa subunit; interleukin 12 polypeptide 35; cytotoxic lymphocyte maturation factor 35 kDa subunit; natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35
products gene name :
IL12A
other gene names :
IL12A; IL12A; IL12p35; Il-12 p35; IL-12A; CLMF p35
uniprot entry name :
IL12A_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine IL12A. No significant cross-reactivity or interference between Bovine IL12A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-12 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-12 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-12 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-12 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_776780.1
ncbi gb acc num :
NM_174355.2
ncbi mol weight :
24,944 Da
ncbi pathways :
African Trypanosomiasis Pathway (194381); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (84358); Allograft Rejection Pathway (535); Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460)
uniprot summary :
Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC
size4 :
10x96-Strip-Wells