catalog number :
MBS703897
products type :
ELISA Kit
products full name :
Mouse Platelet Factor 4, PF-4 ELISA Kit
products short name :
[platelet factor 4]
products name syn :
[Mouse Platelet Factor 4; PF-4 ELISA Kit; CXCL4; MGC138298; SCYB4; chemokine (C-X-C motif) ligand 4; platelet factor 4 (chemokine (C-X-C motif) ligand 4) ; Mouse Platelet Factor 4; PF-4]
other names :
[platelet factor 4; Platelet factor 4; platelet factor 4; PF-4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; platelet factor 4; C-X-C motif chemokine 4]
products gene name :
[PF4]
other gene names :
[Pf4; Pf4; Cxcl4; Scyb4; Cxcl4; Scyb4; PF-4]
uniprot entry name :
PLF4_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse pentosidine. No significant cross-reactivity or interference between mouse pentosidine and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for pentosidine has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any pentosidine present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for pentosidine is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of pentosidine bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_064316.1
ncbi gb acc num :
NM_019932.4
ncbi mol weight :
11,243 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway (1001323); Chemokine Receptors Bind Chemokines Pathway (1000944); Chemokine Signaling Pathway (99271); Chemokine Signaling Pathway (672435); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (1000942); Common Pathway (1001321); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318)
uniprot summary :
Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells