catalog number :
MBS703894
products type :
ELISA Kit
products full name :
Mouse growth hormone releasing hormone, GHRH ELISA Kit
products short name :
growth hormone releasing hormone
products name syn :
Mouse growth hormone releasing hormone; GHRH ELISA Kit; GHRF; GRF; MGC119781; OTTHUMP00000030915; growth hormone releasing factor; sermorelin; somatocrinin; somatoliberin; growth hormone releasing hormone
other names :
somatoliberin; Somatoliberin; somatoliberin; GRF; growth hormone-releasing factor; growth hormone-releasing hormone; growth hormone releasing hormone; Growth hormone-releasing factor; GRF; Growth hormone-releasing hormone; GHRH
products gene name :
GHRH
other gene names :
Ghrh; Ghrh; Ghrf; GRF; GHRH
uniprot entry name :
SLIB_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse GHRH. No significant cross-reactivity or interference between Mouse GHRH and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell culture supernates. Detection Range: 0.625 pg/ml-40 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.156 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GHRH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHRH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHRH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_034415.1
ncbi gb acc num :
NM_010285.2
ncbi mol weight :
12,064 Da
ncbi pathways :
Class B/2 (Secretin Family Receptors) Pathway 1000967!!G Alpha (s) Signalling Events Pathway 1000972!!GPCR Downstream Signaling Pathway 1000971!!GPCR Ligand Binding Pathway 1000941!!Glucagon-type Ligand Receptors Pathway 1000969!!Signal Transduction Pathway 970540!!Signaling By GPCR Pathway 971377
uniprot summary :
Function: GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.