ELISA/assay

Human Mucin-7, MUC7 ELISA Kit

MBS703893

48-Strip-Wells

565 USD

ELISA Kit

Human Mucin-7, MUC7 ELISA Kit

mucin 7, secreted

Human Mucin-7; MUC7 ELISA Kit; DKFZp686J03256; FLJ27047; MG2; MGC34772; mucin 7; salivary; mucin 7; secreted

mucin-7; Mucin-7; mucin-7; MUC-7; apo-MG2; salivary mucin-7; mucin 7, salivary; mucin 7, secreted; Apo-MG2; Salivary mucin-7

MUC7

MUC7; MUC7; MG2; MG2; MUC-7

MUC7_HUMAN

Human

377

This assay has high sensitivity and excellent specificity for detection of human MUC7. No significant cross-reactivity or interference between human MUC7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, Cell lysates, saliva aliva aliva. Assay Type: Sandwich. Detection Range: 18.75 ng/ml -1200 ng/ml. Sensitivity: The minimum detectable dose of human MUC7 is typically less than 4.68 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

222418647

NP_001138478.1

NM_001145006.1

Q8TAX7

39,159 Da

Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Termination Of O-glycan Biosynthesis Pathway (530748)

This gene encodes a small salivary mucin, which is thought to play a role in facilitating the clearance of bacteria in the oral cavity and to aid in mastication, speech, and swallowing. The central domain of this glycoprotein contains tandem repeats, each composed of 23 amino acids. The most common allele contains 6 repeats, and some alleles may be associated with susceptibility to asthma. Alternatively spliced transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene.[provided by RefSeq, Feb 2009]

Function: May function in a protective capacity by promoting the clearance of bacteria in the oral cavity and aiding in mastication, speech, and swallowing. Binds P.aeruginosa pili.2 PublicationsManual assertion based on experiment in:Ref.5

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150