catalog number :
MBS703887
products type :
ELISA Kit
products full name :
Rat Angiopoietin 1, ANG-1 ELISA Kit
products short name :
[angiopoietin 1]
products name syn :
[Rat Angiopoietin 1; ANG-1 ELISA Kit; AGP1; AGPT; ANG1; ; angiopoietin 1]
other names :
[angiopoietin-1; Angiopoietin-1; angiopoietin-1; angiopoietin 1]
products gene name :
[ANGPT1]
other gene names :
[Angpt1; Angpt1; Agpt; Agpt1; Ang-1; ANG-1]
uniprot entry name :
ANGP1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat ANGPT1. No significant cross-reactivity or interference between Rat ANGPT1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 1.56 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_445998.1
ncbi gb acc num :
NM_053546.1
ncbi mol weight :
57,461 Da
ncbi pathways :
HIF-1 Signaling Pathway (695233); PI3K-Akt Signaling Pathway (692247); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (869320); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (869318); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)
ncbi summary :
modulates endothelial cell survival and involved in the activation of endothelial cell-specific Tie-2 receptors [RGD, Feb 2006]
uniprot summary :
Function: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells