ELISA/assay

Human defensin, alpha 1, DEFA1 ELISA Kit

MBS703879

48-Strip-Wells

510 USD

ELISA Kit

Human defensin, alpha 1, DEFA1 ELISA Kit

defensin, alpha 1

Human defensin; alpha 1 (DEFA1) ELISA kit; DEF1; DEFA2; HNP-1; HP-1; MGC138393; MRS; OTTHUMP00000196017; defensin; alpha 1; myeloid-related sequence; defensin; alpha 2; myeloid-related sequence; defensin; alpha 1

neutrophil defensin 1; Neutrophil defensin 1; neutrophil defensin 1; defensin, alpha 1B; Defensin, alpha 1; HNP-1; HP-1; HP1

DEFA1

DEFA1B; DEFA1; HP1; HP-1; HNP-1; DEF1; DEFA2; MRS DEFA1B ; HP-1; HP1; HP-2; HP2

DEF1_HUMAN

Human

94

This assay has high sensitivity and excellent specificity for detection of Human DEFA1. No significant cross-reactivity or interference between Human DEFA1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

124248516

NP_001035965.1

NM_001042500.1

P59665

10,201 Da

Alpha-defensins Pathway (530758); Cellular Roles Of Anthrax Toxin Pathway (138076); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Function: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.2 PublicationsManual assertion based on experiment in:Ref.13

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800