catalog number :
MBS703874
products type :
ELISA Kit
products full name :
Dog thrombomodulin, TM ELISA Kit
products short name :
thrombomodulin
products name syn :
Dog thrombomodulin; TM ELISA Kit; AHUS6; CD141; THRM; TM; CD141 antigen; fetomodulin; thrombomodulin
other names :
thrombomodulin; Thrombomodulin; thrombomodulin; CD_antigen: CD141
products gene name :
THBD
other gene names :
THBD; THBD; TM; TM
uniprot entry name :
TRBM_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog TM. No significant cross-reactivity or interference between dog TM and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of dog thrombomodulin(TM) concentrations in serum, plasma, tissue homogenates. Detection Range: 23.44 pg/ml-1500 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of dog TM is typically less than 5.86 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TM. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for TM. The competitive inhibition reaction is launched between with pre-coated TM and TM in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of TM in the samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001006954.1
ncbi gb acc num :
NM_001006953.2
ncbi mol weight :
60,745 Da
ncbi pathways :
Complement And Coagulation Cascades Pathway 84123!!Complement And Coagulation Cascades Pathway 484
uniprot summary :
Function: Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated