ELISA/assay

Human Liver Type Fatty Acid Binding Protein, L-FABP ELISA Kit

MBS703866

96 Strip Wells

760 USD

ELISA Kit

Human Liver Type Fatty Acid Binding Protein, L-FABP ELISA Kit

fatty acid binding protein 1, liver

Human Liver Type Fatty Acid Binding Protein (L-FABP) ELISA KIT; FABPL; L-FABP; Fatty acid-binding protein; liver; fatty acid binding protein 1; liver

fatty acid-binding protein, liver; Fatty acid-binding protein, liver; fatty acid-binding protein, liver; fatty acid-binding protein 1; liver-type fatty acid-binding protein; fatty acid binding protein 1, liver; Fatty acid-binding protein 1; Liver-type fatty acid-binding protein; L-FABP

FABP1

FABP1; FABP1; FABPL; L-FABP; FABPL; L-FABP

FABPL_HUMAN

Human

127

This assay has high sensitivity and excellent specificity for detection of Human FABP1. No significant cross-reactivity or interference between Human FABP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and cell culture supernates. Detection Range: 31.2 pg/ml-2000pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.8 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-FABP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for L-FABP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain L-FABP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of L-FABP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557577

NP_001434.1

NM_001443.2

P07148

14,208 Da

Fat Digestion And Absorption Pathway 194385!!Fat Digestion And Absorption Pathway 194324!!Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway 160977!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!PPAR Signaling Pathway 83042!!PPAR Signaling Pathway 450!!PPARA Activates Gene Expression Pathway 530749!!Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway 119544!!Selenium Metabolism And Selenoproteins Pathway 198846

This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011]

Function: Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.

96 Strip Wells

760 USD