ELISA/assay

Human phospholipid transfer protein, PLTP ELISA Kit

MBS703855

96 Strip Wells

850 USD

ELISA Kit

Human phospholipid transfer protein, PLTP ELISA Kit

phospholipid transfer protein

Human phospholipid transfer protein (PLTP) Elisa Kit; HDLCQ9; lipid transfer protein II; phospholipid transfer protein

phospholipid transfer protein isoform c; Phospholipid transfer protein; phospholipid transfer protein; lipid transfer protein II; BPI fold containing family E; phospholipid transfer protein; Lipid transfer protein II

PLTP

PLTP; PLTP; BPIFE; HDLCQ9

PLTP_HUMAN

Human

398

This assay has high sensitivity and excellent specificity for detection of Human PLTP. No significant cross-reactivity or interference between Human PLTP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 2.34 ng/ml-150 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.58 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Lipid transport

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLTP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLTP bound in the initial step. The color development is stopped and the intensity of the color is measured.

339275807

NP_001229849.1

NM_001242920.1

P55058

54,739 Da

HDL-mediated Lipid Transport Pathway 106158!!Lipid Digestion, Mobilization, And Transport Pathway 106111!!Lipoprotein Metabolism Pathway 106156!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976!!PPAR Signaling Pathway 83042!!PPAR Signaling Pathway 450!!Statin Pathway 198852

The protein encoded by this gene is one of at least two lipid transfer proteins found in human plasma. The encoded protein transfers phospholipids from triglyceride-rich lipoproteins to high density lipoprotein (HDL). In addition to regulating the size of HDL particles, this protein may be involved in cholesterol metabolism. At least two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function: Facilitates the transfer of a spectrum of different lipid molecules, including diacylglycerol, phosphatidic acid, sphingomyelin, phosphatidylcholine, phosphatidylglycerol, cerebroside and phosphatidyl ethanolamine. Essential for the transfer of excess surface lipids from triglyceride-rich lipoproteins to HDL, thereby facilitating the formation of smaller lipoprotein remnants, contributing to the formation of LDL, and assisting in the maturation of HDL particles. PLTP also plays a key role in the uptake of cholesterol from peripheral cells and tissues that is subsequently transported to the liver for degradation and excretion. Two distinct forms of PLTP exist in plasma: an active form that can transfer PC from phospholipid vesicles to high-density lipoproteins (HDL), and an inactive form that lacks this capability.

96 Strip Wells

850 USD