ELISA/assay

Mouse Ceruloplasmin, CP/CER ELISA Kit

MBS703852

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Ceruloplasmin, CP/CER ELISA Kit

[ceruloplasmin (ferroxidase)]

[Mouse Ceruloplasmin; CP/CER ELISA Kit; CP-2; ceruloplasmin; ceruloplasmin (ferroxidase)]

[ceruloplasmin isoform c; Ceruloplasmin; ceruloplasmin; ferroxidase; ceruloplasmin; Ferroxidase]

[CP]

[Cp; Cp; D3Ertd555e]

CERU_MOUSE

Mouse

1085

This assay has high sensitivity and excellent specificity for detection of human citrulline. No significant cross-reactivity or interference between human citrulline and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma. Assay Type: Competitive. Detection Range: 1.25 nmol/ml-100 nmol/ml. Sensitivity: 1.56nmol/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with citrulline antibody. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated citrulline. The competitive inhibition reaction is launched between with HRP labeled citrulline and unlabeled citrulline with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of citrulline in the sample. The color development is stopped and the intensity of the color is measured.

442745912

NP_001263177.1

NM_001276248.1

Q61147

121,151 Da

Iron Uptake And Transport Pathway (1001366); Metal Ion SLC Transporters Pathway (1001350); Porphyrin And Chlorophyll Metabolism Pathway (83219); Porphyrin And Chlorophyll Metabolism Pathway (407); SLC-mediated Transmembrane Transport Pathway (970707); Transmembrane Transport Of Small Molecules Pathway (970706); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (1001343)

The protein encoded by this gene is a copper-containing glycoprotein found soluble in the serum and GPI-anchored in other tissues. It oxidizes Fe(II) to Fe(III) and is proposed to play an important role in iron homeostasis. In humans mutations of this gene cause aceruloplasminemia, which is characterized by retinal degeneration, diabetes, anemia and neurological symptoms. In mouse deficiency of this gene in combination with a deficiency of its homolog hephaestin causes retinal degeneration and serves as a pathophysiological model for aceruloplasminemia and age-related macular degeneration. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

Function: Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe2+ to Fe3+ without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu2+ ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense . Catalytic activity: 4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O. Cofactor: Binds 6 copper ions per monomer

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

575

96-Strip-Wells

835

5x96-Strip-Wells

3040

10x96-Strip-Wells

5755