ELISA/assay

Mouse osteonectin, ON ELISA Kit

MBS703847

96 Strip Wells

895 USD

ELISA Kit

Mouse osteonectin, ON ELISA Kit

secreted protein, acidic, cysteine-rich (osteonectin)

Mouse osteonectin; ON ELISA Kit; ON; cysteine-rich protein; osteonectin; secreted protein; acidic; cysteine-rich; secreted protein; acidic; cysteine-rich (osteonectin)

SPARC; SPARC; SPARC; osteonectin; basement-membrane protein 40; secreted protein acidic and rich in cysteine; secreted acidic cysteine rich glycoprotein; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine

SPARC

Sparc; Sparc; ON; BM-40; BM-40; ON

SPRC_MOUSE

Mouse

302

This assay has high sensitivity and excellent specificity for detection of Mouse SPARC. No significant cross-reactivity or interference between Mouse SPARC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 31.2 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 8 pg/ml

Sample Volume: 50-100ul

Introduction: Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. Some current research shows a correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis. Osteonectin is an acidic, secreted extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. Additional functions of osteonectin beneficial to tumor cells include angiogenesis, proliferation and migration. Overexpression of osteonectin is reported in many Mouse cancers such as breast, prostate and colon. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ON. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ON and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ON in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6678077

NP_033268.1

NM_009242.5

P07214

34,450 Da

Binding And Uptake Of Ligands By Scavenger Receptors Pathway 1000591!!ECM Proteoglycans Pathway 1001027!!Extracellular Matrix Organization Pathway 1001015!!Hemostasis Pathway 1001295!!Platelet Activation, Signaling And Aggregation Pathway 1001305!!Platelet Degranulation Pathway 1001317!!PodNet: Protein-protein Interactions In The Podocyte Pathway 755428!!Response To Elevated Platelet Cytosolic Ca2+ Pathway 1001315!!Scavenging By Class H Receptors Pathway 1000596!!Senescence And Autophagy Pathway 198361

Function: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity.

96 Strip Wells

895 USD