ELISA/assay

Rat pigment epithelium-derived factor, PEDF ELISA Kit

MBS703843

96 Strip Wells

850 USD

ELISA Kit

Rat pigment epithelium-derived factor, PEDF ELISA Kit

pigment epithelium-derived factor, PEDF

Rat pigment epithelium-derived factor; PEDF ELISA Kit; pigment epithelium-derived factor; PEDF

pigment epithelium-derived factor; Pigment epithelium-derived factor; pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1

PEDF

SERPINF1; SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35; PEDF; PIG35; PEDF

PEDF_HUMAN

Rat

418

This assay has high sensitivity and excellent specificity for detection of rat PEDF. No significant cross-reactivity or interference between rat PEDF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat pigment epithelium-derived factor (PEDF) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 6.25 pg/ml - 400 pg/ml.

Sensitivity: The minimum detectable dose of rat PEDF is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: </b<This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEDF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEDF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEDF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEDF bound in the initial step. The color development is stopped and the intensity of the color is measured.

39725934

NP_002606.3

NM_002615.5

P36955

46,312 Da

The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]

Function: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.2 PublicationsManual assertion based on experiment in:Ref.13

96 Strip Wells

850 USD