ELISA/assay

Pig hepatocyte growth factor, HGF ELISA Kit

MBS703830

48-Strip-Wells

565 USD

ELISA Kit

Pig hepatocyte growth factor, HGF ELISA Kit

hepatocyte growth factor, HGF

Pig hepatocyte growth factor; HGF ELISA Kit; hepatocyte growth factor; HGF

hepatocyte growth factor isoform 3; Hepatocyte growth factor; hepatocyte growth factor; hepatopoeitin-A; hepatopoietin-A; lung fibroblast-derived mitogen; fibroblast-derived tumor cytotoxic factor; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoietin-A; Scatter factor; SF

HGF

HGF; HGF; SF; HGFB; HPTA; F-TCF; DFNB39; HPTA; SF

HGF_HUMAN

Pig

723

This assay has high sensitivity and excellent specificity for detection of pig HGF. No significant cross-reactivity or interference between pig HGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: Typically less than 15.6 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

58533170

NP_001010932.1

NM_001010932.1

P14210

24,116 Da

Arf6 Signaling Events Pathway (138034); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Direct P53 Effectors Pathway (137939); FGF Signaling Pathway (137989); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); Hemostasis Pathway (106028)

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.2 PublicationsManual assertion based on experiment in:Ref.23

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150