Pig matrix metalloproteinase 9, MMP-9 ELISA Kit | MyBioSource MBS703816 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Pig matrix metalloproteinase 9, MMP-9 ELISA Kit

catalog :

MBS703816

quantity :

48-Strip-Wells

price :

490 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703816

products type :

ELISA Kit

products full name :

Pig matrix metalloproteinase 9, MMP-9 ELISA Kit

products short name :

matrix metalloproteinase 9, MMP-9

products name syn :

Pig matrix metalloproteinase 9; MMP-9 ELISA Kit; matrix metalloproteinase 9; MMP-9

other names :

matrix metalloproteinase-9 preproprotein; Matrix metalloproteinase-9; matrix metalloproteinase-9; 92 kDa gelatinase; type V collagenase; macrophage gelatinase; 92 kDa type IV collagenase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

products gene name :

MMP-9

other gene names :

MMP9; MMP9; GELB; CLG4B; MMP-9; MANDP2; CLG4B; MMP-9; GELB

uniprot entry name :

MMP9_HUMAN

reactivity :

Pig

sequence length :

707

specificity :

This assay has high sensitivity and excellent specificity for detection of pig MMP-9. No significant cross-reactivity or interference between pig MMP-9 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

74272287

ncbi acc num :

NP_004985.2

ncbi gb acc num :

NM_004994.2

uniprot acc num :

P14780

ncbi mol weight :

78,458 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Angiogenesis Pathway (198772); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Axon Guidance Pathway (105688); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); CXCR4-mediated Signaling Events Pathway (137910); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly- -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.1 PublicationManual assertion based on experiment in:Ref.16. Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V.1 PublicationManual assertion based on experiment in:Ref.16. Cofactor: Binds 2 zinc ions per subunit. Enzyme regulation: Inhibited by histatin-3 1/24 (histatin-5). Inhibited by ECM1.2 PublicationsManual assertion based on experiment in:Ref.19

size1 :

48-Strip-Wells

price1 :

490 USD

size2 :

96-Strip-Wells

price2 :

690

size3 :

5x96-Strip-Wells

price3 :

2560

size4 :

10x96-Strip-Wells

price4 :

4790

more info or order :

MyBioSource product webpage