ELISA/assay

Chicken Interferon alpha, IFN-alpha ELISA Kit

MBS703812

48-Strip-Wells

490 USD

ELISA Kit

Chicken Interferon alpha, IFN-alpha ELISA Kit

Interferon alpha, IFN-alpha

Chicken Interferon alpha; IFN-alpha ELISA Kit; Interferon alpha; IFN-alpha

interferon-alpha; Interferon omega-1; interferon omega-1; interferon alpha-II-1; IFN-omega 1, interferon omega-1; interferon, omega 1; Interferon alpha-II-1

IFN-alpha

IFNW1; IFNW1

IFNW1_HUMAN

Chicken

195

This assay has high sensitivity and excellent specificity for detection of chicken IFN-alpha. No significant cross-reactivity or interference between chicken IFN-alpha and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of chicken IFN-alpha is typically less than 0.78 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

386800

AAA52724.1

P05000

22,319 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)

IFNW1: Belongs to the alpha/beta interferon family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 9p22. Cellular Component: extracellular space. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor binding; interferon-alpha/beta receptor binding; cytokine activity. Biological Process: B cell proliferation; adaptive immune response; response to exogenous dsRNA; cytokine and chemokine mediated signaling pathway; response to virus; B cell differentiation; natural killer cell activation during immune response; regulation of MHC class I biosynthetic process; innate immune response; cell cycle arrest; defense response to virus; humoral immune response; T cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein

48-Strip-Wells

490 USD

96-Strip-Wells

690

5x96-Strip-Wells

2560

10x96-Strip-Wells

4790