ELISA/assay

Human Thymosin beta4 ELISA Kit

MBS703805

48-Strip-Wells

565 USD

ELISA Kit

Human Thymosin beta4 ELISA Kit

thymosin beta 4, X-linked

Human Thymosin beta4 ELISA Kit; FX; PTMB4; TB4X; TMSB4; OTTHUMP00000022925; OTTHUMP00000022927; OTTHUMP00000022928; prothymosin beta-4; thymosin beta-4; thymosin; beta 4; thymosin; beta 4; X chromosome; thymosin beta 4; X-linked

thymosin beta-4; Thymosin beta-4; thymosin beta-4; t beta-4; prothymosin beta-4; thymosin, beta 4, X chromosome; thymosin beta 4, X-linked; FxCleaved into the following chain:Hematopoietic system regulatory peptideAlternative name(s):Seraspenide

TMSB4X

TMSB4X; TMSB4X; FX; TB4X; PTMB4; TMSB4; TB4X; THYB4; TMSB4; T beta-4

TYB4_HUMAN

Human

44

This assay has high sensitivity and excellent specificity for detection of human thymosin beta4. No significant cross-reactivity or interference between human thymosin beta4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 78 ng/ml -5000 ng/ml. Sensitivity: The minimum detectable dose of human thymosin beta4 is typically less than 19.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for thymosin beta4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any thymosin beta4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for thymosin beta4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of thymosin beta4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

11056061

NP_066932.1

NP_066932.1

P62328

5,053 Da

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Regulation Of Actin Cytoskeleton Pathway (198874); Regulation Of Actin Cytoskeleton Pathway (83089); Regulation Of Actin Cytoskeleton Pathway (500); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048)

This gene encodes an actin sequestering protein which plays a role in regulation of actin polymerization. The protein is also involved in cell proliferation, migration, and differentiation. This gene escapes X inactivation and has a homolog on chromosome Y. [provided by RefSeq, Jul 2008]

Function: Plays an important role in the organization of the cytoskeleton Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150