Human indoleamine 2, 3-dioxygenase, IDO ELISA Kit | MyBioSource MBS703797 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human indoleamine 2, 3-dioxygenase, IDO ELISA Kit

catalog :

MBS703797

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703797

products type :

ELISA Kit

products full name :

Human indoleamine 2, 3-dioxygenase, IDO ELISA Kit

products short name :

indoleamine 2, 3-dioxygenase 1

products name syn :

Human indoleamine 2; 3-dioxygenase; IDO ELISA Kit; CD107B; IDO; INDO; indole 2; 3-dioxygenase; indoleamine-pyrrole 2; 3 dioxygenase; indoleamine 2; 3-dioxygenase 1

other names :

indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; indole 2,3-dioxygenase; indolamine 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase

products gene name :

IDO1

other gene names :

IDO1; IDO1; IDO; INDO; IDO-1; IDO; INDO; IDO-1

uniprot entry name :

I23O1_HUMAN

reactivity :

Human

sequence length :

403

specificity :

This assay has high sensitivity and excellent specificity for detection of human IDO. No significant cross-reactivity or interference between human IDO and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml-50 ng/ml. Sensitivity: Typically less than 0.195 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IDO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IDO bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4504577

ncbi acc num :

NP_002155.1

ncbi gb acc num :

NM_002164.5

uniprot acc num :

P14902

ncbi mol weight :

45,326 Da

ncbi pathways :

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); NAD Biosynthesis II (from Tryptophan) Pathway (138368); Tryptophan Catabolism Pathway (106190); Tryptophan Metabolism Pathway (82964); Tryptophan Metabolism Pathway (198850); Tryptophan Metabolism Pathway (332)

ncbi summary :

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

uniprot summary :

Function: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.1 PublicationManual assertion based on experiment in:Ref.7. Catalytic activity: D-tryptophan + O2 = N-formyl-D-kynurenine.1 PublicationManual assertion based on experiment in:Ref.7. Cofactor: Binds 1 heme group per subunit. Enzyme regulation: Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan).1 PublicationManual assertion based on experiment in:Ref.7

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage