Human decay-accelerating factor, DAF/CD55 ELISA Kit | MyBioSource MBS703778 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human decay-accelerating factor, DAF/CD55 ELISA Kit

catalog :

MBS703778

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703778

products type :

ELISA Kit

products full name :

Human decay-accelerating factor, DAF/CD55 ELISA Kit

products short name :

CD55 molecule, decay accelerating factor for complement (Cromer blood group)

products name syn :

Human decay-accelerating factor (DAF/CD55) ELISA Kit; RP11-357P18.1; CR; CROM; DAF; TC; CD55 antigen; decay accelerating factor for complement; CD55 molecule; decay accelerating factor for complement (Cromer blood group)

other names :

complement decay-accelerating factor isoform 1 preproprotein; Complement decay-accelerating factor; complement decay-accelerating factor; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); CD_antigen: CD55

products gene name :

CD55

other gene names :

CD55; CD55; CR; TC; DAF; CROM; CR; DAF

uniprot entry name :

DAF_HUMAN

reactivity :

Human

sequence length :

381

specificity :

This assay has high sensitivity and excellent specificity for detection of human DAF/CD55. No significant cross-reactivity or interference between human DAF/CD55 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.6 25 ng/ml -40 ng/ml. Sensitivity: The minimum detectable dose of human DAF/CD55 is typically less than 0.156 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DAF/CD55 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DAF/CD55 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DAF/CD55 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DAF/CD55 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

10835143

ncbi acc num :

NP_000565.1

ncbi gb acc num :

NM_000574.4

uniprot acc num :

P08174

ncbi mol weight :

41,400 Da

ncbi pathways :

Class B/2 (Secretin Family Receptors) Pathway (106378); Complement Activation, Classical Pathway (198823); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (106405); GPCR Ligand Binding Pathway (161020); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Immune System Pathway (106386); Innate Immune System Pathway (106387)

ncbi summary :

This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins. [provided by RefSeq, Jul 2014]

uniprot summary :

Function: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade.1 PublicationManual assertion based on experiment in:Ref.15

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage