ELISA/assay

Mouse Endothelin 1, ET-1 ELISA Kit

MBS703775

96 Strip Wells

895 USD

ELISA Kit

Mouse Endothelin 1, ET-1 ELISA Kit

endothelin 1

Mouse Endothelin 1; ET-1 ELISA Kit; ET1; HDLCQ7; preproendothelin 1; endothelin 1

endothelin-1; Endothelin-1; endothelin-1; PPET1; preproendothelin; preproendothelin-1; endothelin 1; Preproendothelin-1; PPET1

EDN1

Edn1; Edn1; ET-1; preproET; ET-1; PPET1

EDN1_MOUSE

Mouse

202

This assay has high sensitivity and excellent specificity for detection of Mouse EDN1. No significant cross-reactivity or interference between Mouse EDN1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul

Introduction: Endothelin-1(ET-1), a peptide of 21 amino acid residues, is the most potent vasoconstrictive substance known. Originally isolated from porcine aortic endothelial cells, ET-1 is now known to be one of a family of three mammalian vasoactive peptides that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3). These related peptides differ from ET-1 at two and six amino acid residue positions, respectively. A fourth peptide, vasoactive intestinal contractor (VIC), is sometimes classified as rat ET-2. All members of the endothelin family contain two essential disulfide bridges and six conserved amino acid residues at the C-terminus. Additionally, all of the endothelin family members are synthesized initially as prepropolypeptides of approximately 200 amino acid residues encoded by separate genes. These are proteolytically cleaved to produce biologically-inactive propolypeptides of approximately 40 amino acid residues termed "big endothelins". Big ET-1 is cleaved by the proteolytic action of a membrane-bound metalloprotease [endothelin-converting enzyme (ECE-1)] producing the 21 amino acid residue active peptide. The biochemistry and biology of the endothelins have been the subject of several reviews. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ET-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ET-1. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ET-1. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6753720

NP_034234.1

NM_010104.3

P22387

22,809 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!G Alpha (q) Signalling Events Pathway 1000975!!GPCR Downstream Signaling Pathway 1000971!!GPCR Ligand Binding Pathway 1000941!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 970815!!HIF-1 Signaling Pathway 695223!!Melanogenesis Pathway 83289!!Melanogenesis Pathway 504!!MicroRNAs In Cardiomyocyte Hypertrophy Pathway 198337!!Peptide Ligand-binding Receptors Pathway 1000943

Function: Endothelins are endothelium-derived vasoconstrictor peptides.

96 Strip Wells

895 USD