catalog number :
MBS703771
products type :
ELISA Kit
products full name :
Mouse N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit
products short name :
N-acetyl-beta-D-glucosaminidase, NAG
products name syn :
Mouse N-acetyl-beta-D-glucosaminidase; NAG ELISA Kit; ENC-1AS; N-acetyl-beta-glucosaminidase; hexosaminidase B; Beta-hexosaminidase subunit beta (HEXB) ; N-acetyl-beta-D-glucosaminidase; NAG
other names :
beta-hexosaminidase subunit beta; Beta-hexosaminidase subunit beta; beta-hexosaminidase subunit beta; hexosaminidase subunit B; beta-N-acetylhexosaminidase subunit beta; N-acetyl-beta-glucosaminidase subunit beta; hexosaminidase B; Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
other gene names :
Hexb; Hexb; Hexosaminidase subunit B
uniprot entry name :
HEXB_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse NAG. No significant cross-reactivity or interference between mouse NAG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 uIU/ml -1000 uIU/ml. Sensitivity: Typically less than 3.9 uIU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_034552.1
ncbi gb acc num :
NM_010422.2
ncbi mol weight :
61,116 Da
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway (83178); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); CS/DS Degradation Pathway (1000660); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1000657); Disease Pathway (970606); Glycogen Storage Diseases Pathway (970607); Glycosaminoglycan Degradation Pathway (83180); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (1000645); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (83196)
uniprot summary :
Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
size4 :
10x96-Strip-Wells