ELISA/assay

Human microtubule-associated protein 2, MAP-2 ELISA Kit

MBS703770

48-Strip-Wells

565 USD

ELISA Kit

Human microtubule-associated protein 2, MAP-2 ELISA Kit

microtubule-associated protein 2

Human microtubule-associated protein 2; MAP-2 ELISA Kit; DKFZp686I2148; MAP2A; MAP2B; MAP2C; OTTHUMP00000163916; microtubule-associated protein 2

microtubule-associated protein 2 isoform 5; Microtubule-associated protein 2; microtubule-associated protein 2; MAP-2; microtubule-associated protein 2

MAP2

MAP2; MAP2; MAP2A; MAP2B; MAP2C; MAP-2

MTAP2_HUMAN

Human

559

This assay has high sensitivity and excellent specificity for detection of human MAP-2. No significant cross-reactivity or interference between human MAP-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: Typically less than 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

87578394

NP_001034627.1

NM_001039538.1

P11137

199,526 Da

LKB1 Signaling Events Pathway (137957); MAPK Cascade Pathway (198834); SIDS Susceptibility Pathways (198901)

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150