Human Slit2 ELISA Kit | MyBioSource MBS703756 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Slit2 ELISA Kit

catalog :

MBS703756

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703756

products type :

ELISA Kit

products full name :

Human Slit2 ELISA Kit

products short name :

slit homolog 2 (Drosophila)

products name syn :

Human Slit2 ELISA Kit; FLJ14420; SLIL3; Slit-2; slit homolog 2; slit homolog 2 (Drosophila)

other names :

slit homolog 2 protein isoform 2; Slit homolog 2 protein; slit homolog 2 protein; slit homolog 2 (Drosophila)

products gene name :

SLIT2

other gene names :

SLIT2; SLIT2; SLIL3; Slit-2; SLIL3; Slit-2

uniprot entry name :

SLIT2_HUMAN

reactivity :

Human

sequence length :

1525

specificity :

This assay has high sensitivity and excellent specificity for detection of human Slit2. No significant cross-reactivity or interference between human Slit2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml -50 ng/ml. Sensitivity: 0.195 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Slit2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Slit2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Slit2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Slit2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

574281850

ncbi acc num :

NP_001276064.1

ncbi gb acc num :

NM_001289135.1

uniprot acc num :

O94813

ncbi mol weight :

169,870 Da

ncbi pathways :

Activation Of Rac Pathway (119530); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Glypican 1 Network Pathway (138010); Inactivation Of Cdc42 And Rac Pathway (119529); Netrin-1 Signaling Pathway (160999); Regulation Of Commissural Axon Pathfinding By Slit And Robo Pathway (119528); Role Of Abl In Robo-Slit Signaling Pathway (119531)

ncbi summary :

This gene encodes a member of the slit family of secreted glycoproteins, which are ligands for the Robo family of immunoglobulin receptors. Slit proteins play highly conserved roles in axon guidance and neuronal migration and may also have functions during other cell migration processes including leukocyte migration. Members of the slit family are characterized by an N-terminal signal peptide, four leucine-rich repeats, nine epidermal growth factor repeats, and a C-terminal cysteine knot. Proteolytic processing of this protein gives rise to an N-terminal fragment that contains the four leucine-rich repeats and five epidermal growth factor repeats and a C-terminal fragment that contains four epidermal growth factor repeats and the cysteine knot. Both full length and cleaved proteins are secreted extracellularly and can function in axon repulsion as well as other specific processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.6 PublicationsManual assertion based on experiment in:Ref.3

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage