Human granzymes B, Gzms-B ELISA Kit | MyBioSource MBS703753 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human granzymes B, Gzms-B ELISA Kit

catalog :

MBS703753

quantity :

48-Strip-Wells

price :

370 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS703753

products type :

ELISA Kit

products full name :

Human granzymes B, Gzms-B ELISA Kit

products short name :

granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)

products name syn :

Human granzymes B; Gzms-B ELISA Kit; CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; HLP; SECT; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic serine protease B; fragmentin 2; granzyme B; granzyme B (granzyme 2; cytotoxic T-lymphocyte-associated serine esterase 1)

other names :

granzyme B; Granzyme B; granzyme B; C11; CTLA-1; fragmentin 2; fragmentin-2; cathepsin G-like 1; human lymphocyte protein; T-cell serine protease 1-3E; cytotoxic serine protease B; cytotoxic T-lymphocyte proteinase 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); C11; CTLA-1; Cathepsin G-like 1; CTSGL1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Granzyme-2; Human lymphocyte protein; HLP; SECT; T-cell serine protease 1-3E

products gene name :

GZMB

other gene names :

GZMB; GZMB; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; CGL1; CSPB; CTLA1; GRB; CTSGL1; Lymphocyte protease; HLP

uniprot entry name :

GRAB_HUMAN

reactivity :

Human

sequence length :

247

specificity :

This assay has high sensitivity and excellent specificity for detection of human Gzms-B. No significant cross-reactivity or interference between human Gzms-B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human Gzms-B is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Gzms-B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Gzms-B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Gzms-B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Gzms-B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

221625528

ncbi acc num :

NP_004122.2

ncbi gb acc num :

NM_004131.4

uniprot acc num :

P10144

ncbi mol weight :

27,716 Da

ncbi pathways :

Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway (105657); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Apoptosis Pathway (198797); Apoptosis Pathway (105648); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Caspase Cascade In Apoptosis Pathway (137974); Downstream Signaling In Naive CD8+ T Cells Pathway (138018)

ncbi summary :

Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. Catalytic activity: Preferential cleavage: -Asp- -Xaa- >> -Asn- -Xaa- > -Met- -Xaa-, -Ser- -Xaa-.1 PublicationManual assertion based on experiment in:Ref.12. Enzyme regulation: Inactivated by the serine protease inhibitor diisopropylfluorophosphate.1 PublicationManual assertion based on experiment in:Ref.12

size1 :

48-Strip-Wells

price1 :

370 USD

size2 :

96-Strip-Wells

price2 :

525

size3 :

5x96-Strip-Wells

price3 :

2025

size4 :

10x96-Strip-Wells

price4 :

3775

more info or order :

MyBioSource product webpage