ELISA/assay

Rat Pentosidine ELISA Kit

MBS703744

96 Strip Wells

850 USD

ELISA Kit

Rat Pentosidine ELISA Kit

Pentosidine

Rat Pentosidine ELISA Kit; Pentosidine

Pentosidine

Rat

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 15.6 pmol/ml-1000 pmol/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pmol/ml

Sample Volume: 50-100ul

Introduction: Pentosidine is a biomarker for advanced glycation endproducts, or AGEs. It is a well characterized and easily detected member of this large class of compounds. AGEs are biochemicals formedcontinuously under normal circumstances, but more rapidly under a variety of stresses, especially oxidative stress and hyperglycemia. They serve as markers of stress and act as toxins themselves. Pentosidine is typical of the class, except that it fluoresces, which allows it to be seen and measured easily. Because it is well characterized, it is often studied to provide new insight into the biochemistry of AGE compounds in general. Derived from ribose, a pentose, pentosidine forms fluorescent cross-links between the arginine and lysine residues in collagen. It is formed in a reaction of the amino acids with the Maillard reaction products of ribose. Although it is present only in trace concentrations among tissue proteins, it is useful for assessing cumulative damage to proteins-advanced glycation endproducts-by non-enzymatic browning reactions with carbohydrates. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to pentosidine. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for pentosidine and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain pentosidine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of pentosidine in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD